The sequence of a viral double-stranded DNA is shown below. This DNA fragment en

Question

The sequence of a viral double-stranded DNA is shown below. This DNA fragment encodes two viral peptides VP1 and VP2. VP1 is encoded by the coding strand and VP2 is encoded by the complementary strand. Each of the genes encoding for the two peptides has a starting cordon and a stop codon. Addign of this double stranded DNA to an in viro transcription and translation system yields two peptides of 5 residues (VP1) and 10 residues (VP2). 1 10 20 30 40 5' AGATCGGATGCTCAACTATATATGTGATTAACAGAGCATGCGGCATAAACT 3' 3' TCTAGCCTACGAGTTGATATACACTAATTGTCTCGTACGCCGTATTTGA 5' Identify the DNA sequence that code for each of the peptides. Determine the amino acid sequence of each peptide according to the genetic code table provided. Please draw the structure of VP1. Label each amino acid with their full name and one letter addreviation. In a mutant viral strain, the T at position 23 has been replaced with G. Determine the amino acid sequence of the peptides encoded by the mutant virus.

Explanation / Answer

A)

3’- TAC GAG TTG ATA TAC- 5’ (AP1 ; 8 - 22)

3’ - TAC GGC GTA CGA GAC AAT TAG TGT ATA TCA - 5’ (AP2 ; 43 - 15)

B & C)

AP1: mRNA sequence 5' - ATG CUC AAC UAU AUG - 3'

protein sequence Met – Leu – Asn – Ile – Met

M – L – N – I - M

AP2: mRNA sequence 5' - AUG CCG CAU GCU CUG UUA AUC ACA UAU AGU - 3'

protein sequence Met – Pro – His – Ala – Leu – Leu – Ile – Thr – Tyr – Ser

M – P – H – A – L – L – I – T – Y – S

D)

AP1 mutant: Met – Leu – Asn – Ile – Met – Pro – Asn – Cys – Leu – Val – Arg – Arg – Ile

AP2 mutant: Met – Pro – His – Ala – Leu – Leu – Ile – Pro – Tyr – Ser

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