A fragment of a wild-type polypeptide is sequenced for seven amino acids. The sa

Question

A fragment of a wild-type polypeptide is sequenced for seven amino acids. The same polypeptide region is sequenced in four mutants. Identify the mutations by matching the number to the correct type of mutation:

Wild-type N . . . Thr-His-Ser-Gly-Leu-Lys-Ala . . . C polypeptide

Mutant 1 N . . . Thr-His-Ser-Val-Leu-Lys-Ala . . . C

Mutant 2 N. . . Thr-His-Ser-C

Mutant 3 N . . . Thr-Thr-Leu-Asp-C

Mutant 4 N . . . Thr-Gln-Leu-Trp-Ile-Glu-Gly . . . C

Are the mutations ( A. Missense B. Nonsense C. Deletion D. Insertion)

Explanation / Answer

Mutant 1 is Misense mutation (option A), Gly in WT to Val is the mutation (a single nucleotide change results in a codon that codes for a different amino acid)

Mutant 2 is nonsense mutation (option B), premature stop codon results in truncated protein

Mutant 3 (A and B are true) (polypeptide is truncaated and different amino acids)

Mutant 4 A is true (number of amino acids are same compare to WT but coded for different aminoacids)

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