Vitamin E is the name for a set of chemically related tocopherols, which are lip
ID: 72659 • Letter: V
Question
Vitamin E is the name for a set of chemically related tocopherols, which are lipid-soluble compounds with antioxidant properties. Such antioxidants protect cells against the effects of free radicals created as by-products of energy metabolism in the mitochondrion. Different tocopherols have different biological activities due to differences in their retention by binding to gut proteins during digestion. The one retained at the highest level is a-tocopherol, while y-tocopherol is retained at less than 10% of that efficiency. In Arabidopsis, alpha-tocopherol is the most abundant tocopherol in leaves, while y-tocopherol is the most abundant in seeds. An enzyme encoded by the VTE4 gene can convert y-tocopherol to alpha-tocopherol. How would you create an Arabidopsis plant that produces high levels of alpha-tocopherol in the seeds?Explanation / Answer
A. B,)Ac DNA of the VTE4 gene can be inserted in to bacterial plasmid which is most efficient vector of r-DNA technology , the resulting construct can be introduced in to bacteria by transformation .
The VTE4 protien can be collected from culture media and used to treat Arabidopsis.
By these techniq bases on the VTE4 gene property Arabidopsis plant that produces high level of alpha -tocopherol in the seeds.
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