1.) You have 100 ml each of 400 mg/ml sodium phosphate, 250 mg/ml sodium chlorid
ID: 7213 • Letter: 1
Question
1.) You have 100 ml each of 400 mg/ml sodium phosphate, 250 mg/ml sodium chloride, and 100 mg/ml potassium nitrate. You want to make one liter of a solution containing 10 mg/ml sodium phosphate, 10 mg/ml sodium chloride, and 10 mg/ml potassium nitrate. How much of each starting solution do you need to use, and how much water do you need to add?2.) You have 100 ml each of 50 mg/ml human chorionic gonadotropin, 25x HCG buffer, and 400 mM potassium chloride. You want to make one liter of a solution that is 1 mg/ml human chorionic gonadotropin, 1x HCG buffer, and 40 mM potassium chloride How much of each starting solution do you need to use, and how much water do you need to add?
Explanation / Answer
you want to make a solution 1000 mls containing 10mg/ml sodium phosphate, 10 mg/ml sodium chloride, and 10 mg/ml potassium nitrate
1. Stock NaPo4 400 mg/ml
to make 1000 mls of 10 mg /ml
you will need 10,000 mg or 10000 /400 = 25 mls of Na po4 stock
2. Stock of Nacl 250 mg/ml
to make 1000 ml of 10 mg/ml
you will need 10000 mg or 10000/250 = 40 mls of NaCl stock
3. Stock of PNO3 100 mg/ml
to make 1000 mls of 10 mg /ml
you will need 10,000 mg or 10000 /1000 = 100 mls of PNO3 stock
take 25 mls of Na po4 stock + 40 mls of NaCl stock + 100 mls of PNO3 stock add make it upto 1000 mls with distil water. you will need 835mls of distil water
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