Time, sec Two concentrations (A and B) of the same enzyme were mixed with excess

Question

Time, sec Two concentrations (A and B) of the same enzyme were mixed with excess of substrate in a suitable buffer. The amount of product produced over time from the two concentrations is shown in the above figure. Which concentration (A or B) do you think contains the greater amount of enzyme? Tell me why. The slope of the line from sample "A" had a value of 15 uM of product/sec, and the slope of the line from sample "B" had a value of 3uM 7. f product/sec. If sample "B" contained 22ng of enzyme, how many ng of enzym e would be in sample "A". Explain your reasoning for full points.

Explanation / Answer

Ans 7

Sample A

The equation of the line

Ya = ma * Xa

Product A concentration = slope of A x time taken by A

[Pa] = 15 x ta

Sample B

The equation of the line

Yb = mb * Xb

Product B concentration = slope of B x time taken by B

[Pb] = 3 x tb

Divide [Pa] by [Pb]

[Pa]/[Pb] = 5 x ta/tb

For ta = tb = 1 s

[Pa]/[22] = 5

[Pa] = 110 nb

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