Questions 1. Shown below are 4 TLC plates that have been spotted with a sam and

Question

Questions 1. Shown below are 4 TLC plates that have been spotted with a sam and placed into separate developing chambers. Which plate has been spotued in the correct loeation? 2. What safety precautions should be used when working with ultraviolet (UV) light? 3. How would a developed TLC plate appear if a sample was a mixture of 3 compounds? What is the advantage of using a pencil to draw a line on the TLC plate rather than using a pen? 4. 5. Where is the best place for leaving used capillary pipets and applicators? a on the lab bench b, in the waste paper basket c. in a container reserved for broken glassware 6. Consider a nonpolar compound, N, and a polar compound, P. In a typical TLC experiment using alumina as the stationary phase and hexane as the mobile phase, a which compound will display a higher Rf value? N P or b. which compound will interact more with the alumina? N or P 7. Calculate the Ry value of the compound on the TLC plate shown below. solvent front 2 cm 4 em start I cm

Explanation / Answer

Question 1.

The TLC plate B has been spotted in the correct location.

Explanation:

When you are performing TLC, first of all, you should take a TLC plate, then you should leave some gap below the plate and better to make a straight line above which you need to spot the compound which you want to analyze. Then you have to take a TLC chamber and pour some eluent which is suitable for the selected compound. Now, you have to place the plate into the TLC chamber such that the eluent level should not touch the spotted compound. All these things are matching in the plate B. Hence, plate B is the right answer.

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