Please show how answer was found. Thank you! Following DNA Extraction, once an a

Question

Please show how answer was found. Thank you!

Following DNA Extraction, once an absorbance is obtained, the value is plugged into an equation to determine the concentration of DNA in the sample [DNA] = (A260 * Dr) / 0.02 If you diluted your sample by a factor of 12.0 and obtained an absorbance of 0.0900, what is the DNA concentration of your original sample (in ng/uL)? 54 Given the concentration found above, how much DNA in L would you need to add to your PCR reaction mixture to achieve the desired amount of DNA in solution? 185 How much Sterile Water? (NOTE: Consult Part 2 of the Genetic Markers procedure to find the desired amount of DNA in solution.)

Explanation / Answer

As NOTE given below in the last, that

To find out the desired amount of DNA in solution, we need to consult procedure of the genetic markers.

With the help of this desired value, we can find the amount of DNA need to be added.

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