Hello everyone, I have recently started a biochemistry lab and am having a diffi

Question

Hello everyone, I have recently started a biochemistry lab and am having a difficult time for some reason setting up and understanding the theory behind this question. So I am titrating an amino acid - asparagine. The solution is 50mM in 100mL water. The amount of asparagine is 0.6606g which comes out to be around .005 moles. I now need to determine how many molar equivalents to add to completely protonate the asparagine in the solution. I may just be over thinking this or have a gap in my knowledge as it's been a while since I've dealt with these type problems but as I understand it HCl provides 1 proton to 1 molecule of asparagine. Therefore, it's a 1:1 and I would have to add .005 moles of HCl to the solution? I'm unsure, but hopefully, I could get some clarification on this. Thank you for your time.

Explanation / Answer

The explanation given is somehow correct. But it also depends upon the pH. If pH is neutral then both amino and carboxyl group will be protonated. If solution is strongly acidic then only caboxylic group will be protonated. If it is strongly basic then both groups will remain unprotonated.

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