1. Starting with a 1M stock of NaCl, a researcher prepares a 100 mM working solu
ID: 714278 • Letter: 1
Question
1. Starting with a 1M stock of NaCl, a researcher prepares a 100 mM working solutions. The distillation factor is:2. To prepare 50 mL of a 100 mM solution if NaCl, starting from a 1M stock of NaCl, a researcher should add _____ (volume with units) of the 1M stock to _____ (volume with units) of diluent.
3. A researcher wishes to prepare 200 mL of bacterial culture media with a final concentration of the antibiotic, ampicillin, at 100 ug/mL. The stock solution of ampicillin is 100 mg/ml. The researchers should add ____ (volume with units) of the ampicillin start to _____ (volume with units) of media.
1. Starting with a 1M stock of NaCl, a researcher prepares a 100 mM working solutions. The distillation factor is:
2. To prepare 50 mL of a 100 mM solution if NaCl, starting from a 1M stock of NaCl, a researcher should add _____ (volume with units) of the 1M stock to _____ (volume with units) of diluent.
3. A researcher wishes to prepare 200 mL of bacterial culture media with a final concentration of the antibiotic, ampicillin, at 100 ug/mL. The stock solution of ampicillin is 100 mg/ml. The researchers should add ____ (volume with units) of the ampicillin start to _____ (volume with units) of media.
2. To prepare 50 mL of a 100 mM solution if NaCl, starting from a 1M stock of NaCl, a researcher should add _____ (volume with units) of the 1M stock to _____ (volume with units) of diluent.
3. A researcher wishes to prepare 200 mL of bacterial culture media with a final concentration of the antibiotic, ampicillin, at 100 ug/mL. The stock solution of ampicillin is 100 mg/ml. The researchers should add ____ (volume with units) of the ampicillin start to _____ (volume with units) of media.
Explanation / Answer
1. The stock is diluted to a tenth of the initial concentration. Hence, the distillation factor is 10.
2. 5 ml of the 1M stock to 45 ml of the diluent.
As the volume increases 10 times hence the concentration will decrease to one tenth.
3. As the concentration is to be brought down from 100mg/mL to 100mug/mL, that is one thousandth of the initial, hence the volume must be increased 1000 times that of the initial.
Hence, should add 0.2mL of the ampicillin start to 199.8mL of media.
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