DQuestion 5 1 pts In what order will the following peptides elute from a size ex
ID: 714052 • Letter: D
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DQuestion 5 1 pts In what order will the following peptides elute from a size exclusion chromatography column? l. TYWSFHILAGFTSSIL II. TEDSE III. KAGFELCRNEDACEFKRAKNAQWFSSTIE oll, followed by l, followed by Ill O II, followed by II, followed by O Ill, followed by I, followed by II O Ill, followed by II, followed by I D Question 6 2 pts Sketch a chromatogram for the separation of the three peptides from the size exclusion column in question 5. For this graph, the y-axis is the absorbance at 280 nm and the x-axis is in volume (mL) or time (s) Upload Choose a FileExplanation / Answer
Answer is 3
3 , Followed By 1 , Followed By 2
As the Principle Of size exclusion Principle in the mixture Larger the Conpound It cant stay with the stationary phase and it elute first with mobile phase and the smaller molecules spends more time with the stationary phase so that they elute last..
In our mixture of peptide
peptide number 3 is so bigger and made up of so many different type of amino acid so that it spend very less time in coloum nd elute first than according to size or length of chain of peptide , peptide 1 will be elute and at last peptide 2 will be elute because smaller in size
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