3- In automated (Sanger) sequencing, dATPs are labeled with a green dye, ddTTPs

Question

3- In automated (Sanger) sequencing, dATPs are labeled with a green dye, ddTTPs with a red dye, ddCTPs with a blue dye, and ddGTPs with a yellow dye. In sequencing a 200 bp piece of DNA, a fragment is produced that is 100 nucleotides long and labeled with red. You can conclude that (circle all that apply)

A) The fragment produced (synthesized) contains a T a position 100.

B) The fragment produced (syntehsized) contains an A at position 100.

C) The DNA strand to which the your sequencing primer anneals contains a T a position 100.

D) There are no thymidines present in nucleotides 101-200 of the DNA segment being sequenced.

E) The DNA strand to which the your sequencing primer anneals contains a A a position 100.

A) The fragment produced (synthesized) contains a T a position 100.

B) The fragment produced (syntehsized) contains an A at position 100.

C) The DNA strand to which the your sequencing primer anneals contains a T a position 100.

D) There are no thymidines present in nucleotides 101-200 of the DNA segment being sequenced.

E) The DNA strand to which the your sequencing primer anneals contains a A a position 100.

Explanation / Answer

D) There are no thymidines present in nucleotides 101-200 of the DNA segment being sequenced.

E) The DNA strand to which the your sequencing primer anneals contains a A a position 100.

D) There are no thymidines present in nucleotides 101-200 of the DNA segment being sequenced.

E) The DNA strand to which the your sequencing primer anneals contains a A a position 100.

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