1. You run an LDH assay on one of your samples, and obtain an activity of 1.5 A/
ID: 712119 • Letter: 1
Question
1. You run an LDH assay on one of your samples, and obtain an activity of 1.5 A/min. You then dilute the sample 1:10, and obtain an activity of 1.2 A/min. Is this the activity that you would expect from a 1:10 dilution of your original solution? Why? What is happening in these assays?
2. You obtain a rate of 0.8 A/min from one of your samples (Sample A). You added 10 µl of a 1:10 dilution of Sample A to a total volume of 1.21 ml. What is the corresponding activity (in µmol/min/ml) in the original sample?
Explanation / Answer
1)
LDH assay or Lactate dehydrogenase assay include reactant as NADH and NAD+ as a product.
As activity decreases on performing the dilution because it is required to dilute the enzyme before assay because we want to make sure that diluted sample should have an absorbance which lie between standard curve
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