Chromatin immunoprecipitation technique is used to determine the location of his

Question

Chromatin immunoprecipitation technique is used to determine the location of histone modifications in the genome. How is the key information deciphered from the precipitates?

A) The cross-link between the DNA and histones must remain intact for the analyses.

B) The fragment of DNA bound to modified histones is seperated from histones and sequenced.

C) The fragment of DNA being precipitated is subjected to the DNAse I digest.

D) The DNA methylation patterns are deciphered by removing all methyl groups from DNA fragments.

E) The proteins that are isolated by the immunoprecipitation are subjected to the protease treatment.

Explanation / Answer

B) The fragment of DNA bound to modified histones is seperated from histones and sequenced.

The crosslinked DNA is selectively immunoprecipitated, purified and sequenced in basic chromatin immunoprecipitation technique.

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