Outline an experimental approach to determining the average chain growth rate fo

Question

Outline an experimental approach to determining the average chain growth rate for transcription in vivo. Chain growth rate is the number of nucleotides polymerized per minute per RNA chain.

Drag the terms on the left to the appropriate blanks on the right to complete the sentences.

session.masteringchemistry.com Mastering Chemistry Intro Biochem F15 11:115:301 Help Close Resources us 3 of 76 next » edit mate Problem 24.1 Part A Outine an experimental approach to determining the average chain growth rate for transcription in vivo. Chain growth rate is the number of nucleotides polymerized per minute per RNA chain Drag the terms on the left to the appropriate blanks on the right to complete the sentences ion-exchange chromatography or electrophoresis Label a growing bacterial or cell culture for a brief interval with a mixture of 14C-labeled nucleosides which would be converted to nucleotides and incorporated into . Isolate total alkali and subject it to hydrolysis with mild Every -terminal RNA length sucrose-gradient centrifugation and nucleotide will be released as a nucleoside. Separate nucleosides from nucleotides, by Total picomoles of nucleotide in the hydrolysate divided by total picomoles of nucleoside gives the number of nucleotides incorporated per interval (minutes) gives the growth rate of individual chains (nucleotides incorporated per chain-which, when divided by the labeling quilibrium density-gradient centrifugation chain per minute). An interesting variant of the experiment would be to carry it out acid with individual radiolabeled nucleosides and ask whether the chain is the same for each nucleotide DNA microarravs methods growth rate help

Explanation / Answer

1. DNA
2. RNA

3. alkali

4. 5' terminal nucleoside

5. Sucrose gradient and equilibrium density gradient centrifugation

6. RNA

7. DNA

8. length

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