Table 1 (30 pts.total) Amino Acid Size ranking Polarity d (m) (1-8) Alanine Aspa
ID: 704520 • Letter: T
Question
Table 1 (30 pts.total) Amino Acid Size ranking Polarity d (m) (1-8) Alanine Aspartic acid S Ctycine Leucin serine Trypto pharn 2.50 .om 37 2.4m S37 Tyrosihe m 6.7 60 Unknown spot 1 Unknown spot 2 1. State the unknown code, identify the unknowns and justify reasons for arriving at your conclusion. (4 pts.) the nature of the mobile and stationary phases used in this experiment, describe the size of the amino acid (independent of the polarity) has on the Rr and effect you esve this expectation. You may need to do some research to understand the effect 2. Based on size has in this experiment. (3 pts.) 18Explanation / Answer
1. Unknown code 1 Rf matches well with the Rf value for Leucine. Therefore unknown spot 1 identification is Leucine.
2. As the size of amino acid increases the Rf value also increases. The Rf of alanine is 0.14, whereas for leucine it is 0.60. However the effect is not very consistent for all the amino acids.
3. As the polarity of side chain increases, Rf value decreases. The amino acid stays longer (strong interaction) on the stationary phase and moves slowly with the mobile phase.
4. Tryptophan, tyrosine and aspartic acid have large side chains. The Rf value for tryptophan and tyrosine are about the same. The polatity of aspartic acid side chain reduces the Rf value for it while having string interaction with the stationary phase.
5. Most polar side chain amino acids are aspartic acid, serine and tyrosine. Of these aspartic acid having lowest Rf is most polar of the three. The carboxylic acid group is highly polar, followed by -OH group. The large hydrocarbon ring of tyrosine makes it least polar and more hydrophobic among the three.
6. As the polarity increases, Rf value decreases. Also, as the size of side chain increases Rf value decreases when hydrocarbon chains are considered.
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