Why is the shape of the fluorescence emission spectrum unaffected by a change in

Question

Why is the shape of the fluorescence emission spectrum unaffected by a change in excitation wavelength? How does the emission intensity vary as the excitation wavelength is changed? Why? Compare the fluorescence excitation spectrum with the absorption spectrum of riboflavin. What assumptions are involved in step IID. of the vitamin pill analysis? How can you distinguish a solvent Raman scattering peak from a solute fluorescence peak? scussion: This section will contain a discussion of the results including explanation of any usual data circumstances that might affect interpretation of the data, or comments on

Explanation / Answer

1) the emission of fluorescence always takes place from the lowest vibrational level of the first excited state, the shape of the emission spectrum is always the same, despite changing the wavelength of exciting light.

2)A plot of emission against wavelength for any given excitation wavelength is known as the emission spectrum. If the wavelength of the exciting light is changed and the emission from the sample plotted against the wavelength of exciting light, the result is known as the excitation spectrum. Furthermore, if the intensity of exciting light is kept constant as its wavelength is changed, the plot of emission against exciting wavelength is known as the corrected excitation spectrum.

5) separation can be achieved by changing the excitation wavelength to a lower value; hence the Raman band will also be lowered. Since the wavelength of fluorescence emission is independent of exciting wavelength, the fluorescence will be separated from the Raman scatter.

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