Topic: transcription, translation Can someone please help me answer the question

Question

Topic: transcription, translation

Can someone please help me answer the questions above and explain why did you pick that answer?

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What is the reason that Klenox tragment can rep What is the reason that Klenow fragment can replace PolymeraseI in excision repair nucleotide? What are the major simlarity between the mechanisms between DNA ligase and r similarity between th tRNA synthetase? A bacteria strain has a mutation in protein resistant. What would you target? a) tetracycline. b) c) cycloheximide d) Streptomyces

Explanation / Answer

1). DNA polymerase I is having 5’ to 3’ exonuclease activity, which restricts its use in many biological applications. Klenow fragment is a protein fragment derived by the enzymatic cleavage of DNA polymerase I of E.coli. This fragments retains the polymerase activity in 5’ to 3’ direction and also 3’ to 5’ exonuclease activity, which facilitates proofreading and also removal of precoding nucleotides. Klenow fragment does not contain the 5’ to 3’ exonuclease activity.

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