47. Which of the following factors is important in a theoretical description of
ID: 697749 • Letter: 4
Question
47. Which of the following factors is important in a theoretical description of chromatógraphic behavior? (A) longitudinal (molecular) diffusion (B) flow characteristics of the mobile phase (C) slow equilibrium (D) all of the above 48. The chromatographic peak for a compound was found 13.9 min after sample injection. This peak was Gaussian in shape and had a width at its base of 18.8 s. Calculate the number of theoretical plates for this chromatographic system. (A) 8.7 plates (C) 1.1 × 104 plates (D) 3.1 × 104 plates (B) 2.4 × 103 plates 49. If the length of a chromatographic column is doubled, its chromatographic resolution is increased by a factor of (B) 2.0 (C) 4,0 (D) The resolution is independent of column length.Explanation / Answer
Ans 47 D) All of the above
All of the compounds in chromatography mixture show longitudnal diffusion broadening , which helps them to move in a mobile phase. The stationary and mobile phase never attains equillibrium , if they do so , the efficiency of the system becomes very poor. The movement of the mobile phase helps in efficient seperation of components.
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