Experimental data for RNAse T1 unfolding transition are given in the first two c

Question

Experimental data for RNAse T1 unfolding transition are given in the first two columns of Table 1, T (°C) and ORD measured at 295 nm. The pre

c) Calculate Hm.

d) Cp can be obtained by differential scanning calorimetry. Its value for RNase T1 is about 1250 cal/mol/deg. Calculate G at 15 °C and 30 °C.

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Explanation / Answer

1. Hm Calculation:

Now, we know that

G(T) = Hm (1 - T/Tm) – Cp[(Tm – T) + T ln(T/Tm)]

Now, at Tm, G = 0. Therefore, Hm = (Tm) (Sm)

Or,   Hm = (48.3 + 273.2)(300.3)

Or,    Hm = 96.5 Kcal/mol.

Also, the slope of van’t Hoff plot = -48.631 K.

Therefore, Hm = -(1.987)(-48.631)

Hm = 96.63 Kcal/mol

2. G calculations.

G can be easily calculated using the above equation.

At, the given temperatures of G, assuming Cp = 0, and the substituting the value of given temperature, G can be obtained.

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