For each of the following genotypes, indicate whether beta-galactosidase and per

Question

For each of the following genotypes, indicate whether beta-galactosidase and permease enzymes will be expressed in an inducible (I), non-inducible (N) or constitutive (C) fashion. For example, if a genotype is inducible for beta-gal but constitutive for permease, enter IC. P stands for promoter and is wildtype in all cases. You will have only 2 tries on each genotype. 1) I+P+O+Z-Y+ 2)I+P+O+Z+Y- 3)I-P+O+Z+Y+ 4)I(s)P+O+Z+Y+ 5)I+P+O(c)Z+Y+ 6)I(s) P+ O(c) Z+ Y+ 7)I+ P+ O(c) Z+ Y- 8)I- P+ O+ Z+ Y+ / I+ P+ O+ Z- Y- 9)I- P+ O+ Z+ Y- / I+ P+ O+ Z- Y+ 10)I+ P+ O(c) Z- Y+ / I+ P+ O+ Z+ Y- 11)I(s) P+ O+ Z+ Y- / I+ P+ O+ Z- Y+ 12)I(s) P+ O+ Z+ Y+ / I+ P+ O(c) Z+ Y+

Explanation / Answer

1) I+P+O+Z-Y+ = NI

2)I+P+O+Z+Y- = IN

3)I-P+O+Z+Y+ = CC

4)I(s)P+O+Z+Y+ = NN

5)I+P+O(c)Z+Y+ = CC

6)I(s) P+ O(c) Z+ Y+ = CC

7)I+ P+ O(c) Z+ Y- = IN

8)I- P+ O+ Z+ Y+ / I+ P+ O+ Z- Y- = II

9)I- P+ O+ Z+ Y- / I+ P+ O+ Z- Y+ = II

10)I+ P+ O(c) Z- Y+ / I+ P+ O+ Z+ Y- = CC

11)I(s) P+ O+ Z+ Y- / I+ P+ O+ Z- Y+ = NN

12)I(s) P+ O+ Z+ Y+ / I+ P+ O(c) Z+ Y+ = CC

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