1. As a physician your patient, a 22 year old male, appears to becyanotic. He re
ID: 687118 • Letter: 1
Question
1. As a physician your patient, a 22 year old male, appears to becyanotic. He reports frequent headaches and that he becomes easilyfatigued and out of breath when he exercises. You draw hisblood, which is brownish in color. It does not become red even whenyou shake it with air. You purify his RBCs as well as some skincells and test for the levels of cytochrome B5 reductase. Yourpatient has very low levels of cytochrome B5 reductase in the RBCsbut the level in the skin cells appears to be normal. When yousequence the DNA (gene) for cytochrome B5 reductase, you find thefollowing data:Codon: ---------55--------56------57-------58--------59
Your patient:-----GAC-----ACC---CAG----CGC-----TTC
Normal person: --GAC----ACC----CGC---CGC-----TTC
You have also done asn experiment in which you examine the proteinsstability to heat treatment and find the following data:
%activity remaining after heating at 50degrees C, 80 min:
Normal person: 80
Your patient: 40
Now please interpret all of these data so that you can tell me howthe data account for your patient's symptoms; be sure to explainwhat the problem is and how you know; be sure to indicate themolecular mechanisms of the defects.
2. Calculate the length of a double stranded DNA molecule with a MWof 3x10^5. Also calculate the number of deoxy nucleotide residuesin this molecule. What volume is likely occupied by one molecule ofthis DNA? What assumptions must you make to do thesecalculations?
3. The MW of bacteriophage T4 double stranded DNA is 1.3x10^8. Howmany amino acids could be coded for by the T4 DNA? How manydifferent protein of average MW 55,000 could be coded for by the T4DNA? Show all work and tell me any assumptions you must make to dothese calculations.
4. The following melting temperature (Tm) data were obtained fordouble stranded DNA in 10mM phosphate buffer containing 1 mM EDTA.Please estimate the %G+C content of sample Z. Show all work.
Sample-------------------------------------%G+C------------------Tm(DegreesC)
A-------------------------------------------70.0----------------------78.5
B--------------------------------------------52.5----------------------71.2
C--------------------------------------------37.5----------------------65.0
Z----------------------------------------------?-------------------------73.3
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