1a) Restriction enzymes_______and______ would be used to clone a segment of frog
ID: 68468 • Letter: 1
Question
1a) Restriction enzymes_______and______ would be used to clone a segment of frog DNA.
1b) The size of the recombinant plasmid is _________bp.
1c) The recombinant plasmid when transformed into E. coli confers resistance to which of the following antibiotics:
tetracycline only
ampicillin only
beta-galactocillin only
ampicillin and beta-galactocillin
ampicillin and tetracycline
tetracycline and beta-galactocillin
1d)
The recombinant plasmid map has many restriction sites. Determine the base pair position of each of the following sites in the recombinant plasmid map:
Restriction Enzyme Vector Position Recombinant Position XhoI 240 Answer DraI 935 Answer XmaIII 2780 Answer SacI 3150 AnswerExplanation / Answer
1A. RsaI and ECoRI for cloning the frog DNA
1B. size of recombinant plasmid: 4315bp
1C. ampicillin only. As the Tetr is disturbed due to cloning
1D. DraI= 2100bp
XhoI= 240bp
SmaIII= 3945
SacI= 4315
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