Suppose that each PTK receptor bound to a growth factor converts 190 molecules o

Question

Suppose that each PTK receptor bound to a growth factor converts 190 molecules of Ras protein into GTP-bound form in 2 second and each GTP-bound Ras protein activates 12 Raf kinases in 2 second. Calculate how many active Raf kinases will be produced in 3 seconds after activation of PTK in the absence GAP. Assume that Ras hydrolyses GTP with the rate of 2 molecules/min in the absence of GAP

In rat myocytes the concentration of ATP is 7 mM, ADP is 1 mM and phosphocreatine is ~ 45 mM. What is the highest concentration of creatine that thermodynamically favors for phosphate group to be transferred from phosphocreatine to ADP? Assume T = 310 K.

Compare DGo for hydrolysis of ATP to ADP + Pi with that to AMP + PPi and explain the difference

Explanation / Answer

First let's simplify the question. In the given problem 190 molecules of Ras protein are converted in 2 seconds which means that 95 molecules are converted in 1 second. It is also given that 12 Raf kinases are activated in 2 seconds which means 6 Raf kinesis are activated in 1 second. Now we have to calculate number of active Raf kinases produced in 3 seconds.

First calculating GTP-bound Ras activated:

After 3 seconds = 95 x 3 = 285 molecules

Now, Raf kinases activated after 3 seconds per 1 GTP-bound Ras will be:

6 X 3 = 18 molecules

Thus, calculating Raf kinases produced after 3 seconds

= 285 X 18 = 5130 molecules

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