a) Suppose you have a plasmid that has one site where the restriction enzyme Eco

Question

a) Suppose you have a plasmid that has one site where the restriction enzyme EcoRI cuts. If you digest a sample of many molecules of this plasmid with EcoRI completely, what will you get?

- Many individual DNA nucleotides
- One single DNA molecule
- Many DNA molecules, all of the same length
- Many DNA molecules of different lengths

b)

Based on the lecture, which of the following are true of the Polymerase Chain Reaction (PCR)? Check all that apply. (Lecture with Eric S. Lander on Recombinant DNA III; analyzing a gene)

- It uses an RNA primer
- It requires a special DNA polymerase that works at very low temperatures.
- It makes many copies of a target DNA sequence
- It can start with as little as one DNA molecule
- It involves DNA replication in a living cell

c) At the end of the animation, which of these best describes the DNA molecules present?

- Many copies of the entire DNA molecule and no copies of the target sequence.
- Many copies of the entire DNA molecule and fewer copies of the target sequence.
- Roughly equal numbers of copies of the entire DNA molecule and the target sequence.
- Fewer copies of the entire DNA molecule and more copies of the target sequence.
- Only copies of the target sequence.

Explanation / Answer

a). - Many DNA molecules of different lengths

b). - It uses an RNA primer
- It makes many copies of a target DNA sequence
- It involves DNA replication in a living cell

c). - Many copies of the entire DNA molecule and fewer copies of the target sequence.

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