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Lambda mutations that eliminate the normal functions of cI, cII, and cIII all sh

ID: 66915 • Letter: L

Question

Lambda mutations that eliminate the normal functions of cI, cII, and cIII all show the same phenotype. How could you identify which gene actually has the mutation?

a. mutant mapping using two or three point cross

b. perform complementation tests to determine which is mutated

c. cannot be determined using existing methods

d. both A and b

a. mutant mapping using two or three point cross

b. perform complementation tests to determine which is mutated

c. cannot be determined using existing methods

d. both A and b

Explanation / Answer

Lambda mutations that eliminate the normal functions of cI, cII, and cIII all show the same phenotype. How could you identify which gene actually has the mutation?

The correct statement is...............b. perform complementation tests to determine which is mutated

Mutation in cl , cll. clll give clear plaques showing all cells have lysed.

Every one of these factors are needed for lysogeny.

These proteins can be pooled by chromatography.

cI is wild and it did not bind to DNA of virulent mutants.

A mutated cI grow lytically as it lacks repressor. Accumulation of cII does not affect the lysis decision in the absence of cI.

cI mutation is epistatic to cII mutation.