Suppose you have identified the binding site for a transcription factor that reg

Question

Suppose you have identified the binding site for a transcription factor that regulates the expression of an eukaryotic beta-galactosidase gene. Describe an experiment you might perform to determine if it acts like a proximal element or a distal element (like an enhancer). Assume that you can transform the species, and use the X-Gal method to measure levels of beta-galactosidase. A series of deletions were used to study the promoter of the rice hemi gone, giving the results shown below (the intact promoter is on top). Based on these results, what conclusions could you draw about the nature of the promoter?

Explanation / Answer

4 Q. Nature of the promoter conclusion;

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The regulatory region of the genes in the promoter show relative activity and specificity for the expression level. The series of deletion substantially increased towards the upstream sequences including the enhancer region determines the level of regulatory protein expression and their specificity. The relative activity and specificity of the promoter is evaluated by an assay using linked reporter gene using gene transfection to study the normal effect using cell lines.

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