According to the central dogma, double-stranded DNA serves as the template for t

Question

According to the central dogma, double-stranded DNA serves as the template for the production of RNA during transcription. Which of the two DNA strands serves as the template for transcription?

-Both DNA strands serve as templates simultaneously for RNA polymerase, with continuous transcription on the leading strand and discontinuous transcription on the lagging strand.

-Either DNA strand may be used as a template by RNA polymerase, but a single DNA strand oriented in the 3'–5' direction is used as a template each time transcription occurs.

-The DNA strand oriented in the 5'–3' direction is the only template because only this strand contains the promoter sequences necessary to initiate transcription.

-The DNA strand oriented in the 5'–3' direction is the only template because RNA polymerase synthesizes only in the 5'–3' direction.

Explanation / Answer

d. Either DNA strand may be used as a template by RNA polymerase, but a single DNA strand oriented in the 3'–5' direction is used as a template each time transcription occurs.

During transcription, only the template strand oriented in the 3'–5' direction is used as a template each time transcription occurs. RNA polymerase synthesizes only in the 5'–3' direction and adds nucleotides to the 3' end.

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