Organism Biosafety level Morphological Characteristics Gram +ve or Gram -ve Expe
ID: 66702 • Letter: O
Question
Organism
Biosafety level
Morphological Characteristics
Gram +ve or Gram -ve
Expected Growth Characteristics on Nutrient Agar
Expected Growth Characteristics on EMB Agar
Expected Growth Characteristics on Mannitol Salt Agar
Escherichia coli
Staphylococcus aureus
Pseudomonas aeruginosa
Staphylococcus epidermidis
Organism
Biosafety level
Morphological Characteristics
Gram +ve or Gram -ve
Expected Growth Characteristics on Nutrient Agar
Expected Growth Characteristics on EMB Agar
Expected Growth Characteristics on Mannitol Salt Agar
Escherichia coli
Staphylococcus aureus
Pseudomonas aeruginosa
Staphylococcus epidermidis
Explanation / Answer
Organism Bio safety level Morphological characteristics Gram +ve or -ve Growth characteristics on Nutrient Agar Growth characteristics on EMB Agar Growth characteristics on Mannitol Salt Agar Escherichia coli Level 1 Rods-Non spore forming -ve Ciruclar, raised, small, smooth, shiny, nonpigmented and translucent Produces metallic green color colonies Mannitol positive, produces pink color colonies Staphylococcus aureus Level -2 Cocci in clusters + ve Circular, entire, shiny,large, rough, tan or yellow pigmented and opaque Clear - No acid production and negative for lactose fermentation Does not utilize mannitol. growth will be there but the plate will be yellow in color Pseudomonas aeruginosa Level 2 Rod Gram -ve Circular, entire, raised, surrounded by bluish green color pigment Clear - No acid production and negative for lactose fermentation Mannitol negative. Produces white color colonies Staphyloccus epidermis Level Cocci in single, clusters or as tetrads. + ve Medium sized, white, opaque and flat colonies Clear - No acid production and negative for lactose fermentation Utilizes Mannitol, plate will be pink in color.
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