12. You have a restriction enzyme that cuts once in the middle of a 12KB circula
ID: 66522 • Letter: 1
Question
12. You have a restriction enzyme that cuts once in the middle of a 12KB circular plasmid. What size bands do you expect to see if you run the product(s) of your restriction digest on a gel? Question 12 options:
A) Two bands very close together on the gel
B) One very thick/bright band at 6KB
C) One band at 12KB
D) No bands
The purpose of a mini prep is to:
Question 13 options:
amplify the DNA from bacteria cells
amplify the RNA from bacteria cells
isolate and purify a DNA plasmid from bacteria cells
Insert a DNA plasmid into a bacterial cell
A)amplify the DNA from bacteria cells
B)amplify the RNA from bacteria cells
C)isolate and purify a DNA plasmid from bacteria cells
D)Insert a DNA plasmid into a bacterial cell
Explanation / Answer
12. B) One very thick/bright band at 6KB
If the cut is exactly in the middle then a thick band is seen which indicates the presence of more than one fragment of the same size.
13. isolate and purify a DNA plasmid from bacteria cells
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