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The following is data from a cross of a heterozygous drosophila female and a hom

ID: 65672 • Letter: T

Question

The following is data from a cross of a heterozygous drosophila female and a homozygous recessive male. The data is examining three linked genes. Use the data to determine:

1) The order of the genes on the chromosome.

2) The pattern of the genes on the female’s pair of chromosomes.

3) The map distances between the genes.

Table 1 Drosophila Cross data 1000 F1 progeny scored for 3 linked loci

Trait

number

Smooth abdomen(sa)

3

Lobe Eye(le) and withered wings (ww)

2

Lobe eye (le)

87

withered wings and smooth abdomen

76

Wild type(+, +, +)

390

Lobe eyes, withered wings and smooth abdomen

344

Withered wings

45

Lobe eyes and smooth abdomen

53

Trait

number

Smooth abdomen(sa)

3

Lobe Eye(le) and withered wings (ww)

2

Lobe eye (le)

87

withered wings and smooth abdomen

76

Wild type(+, +, +)

390

Lobe eyes, withered wings and smooth abdomen

344

Withered wings

45

Lobe eyes and smooth abdomen

53

Explanation / Answer

1. From the double crossover we can say that the order of genes is ww, sa, le.

2.

Trihybrid cross

S. No.

Genotype

Number

Progeny type

1

Smooth abdomen(sa)

3

Double crossover

2

Lobe Eye(le) and withered wings (ww)

2

Double crossover

3

Lobe eye (le)

87

Single cross over

4

withered wings and smooth abdomen

76

Single cross over

5

Wild type(+, +, +)

390

Parental

6

Lobe eyes, withered wings and smooth abdomen

344

Parental

7

Withered wings

45

Single cross over

8

Lobe eyes and smooth abdomen

53

Single cross over

1). From the double crossover, we can say that these genes are in the order of ww sa le.

3). The gene distance between ww and sa loci is = (3+45+53+2/1000) *100 = 10.3 m.u.

Distance between le and ww loci = (3+ 87+ 76 +2/ 1000)* 100 = 16.8   = 16.8 m.u (map units).

Now, the distance between ww and le loci = 10.3 + 16.8 = 27.1 map units

S. No.

Genotype

Number

Progeny type

1

Smooth abdomen(sa)

3

Double crossover

2

Lobe Eye(le) and withered wings (ww)

2

Double crossover

3

Lobe eye (le)

87

Single cross over

4

withered wings and smooth abdomen

76

Single cross over

5

Wild type(+, +, +)

390

Parental

6

Lobe eyes, withered wings and smooth abdomen

344

Parental

7

Withered wings

45

Single cross over

8

Lobe eyes and smooth abdomen

53

Single cross over