To test if MAPK was activated by BDNF, wild type neurons were treated with BDNF,

Q: To test if MAPK was activated by BDNF, wild type neurons were treated with BDNF,

9. 10. PD had no effect on the percentage of neurons with BDNF-stimulated filopodia 11. MAPK inhibition is required for filopodia formation A) Inhibited MAPK

ID: 65607 • Letter: T

Question

To test if MAPK was activated by BDNF, wild type neurons were treated with BDNF, or left untreated (UN). Cells were lysed and the proteins separated by SDS-PAGE. Phosphorylated MAPK (p-MAPK) and the NR1 subunit of the glutamate receptor (loading control) were visualized by western/immuno blotting.

Question 9

What did BDNF do to MAPK in these neurons?

D) Activated MAPK

To test if MAPK played a role in formation of BDNF-stimulated axonal filopodia, a MEK inhibitor was used (PD). MEK phosphorylates and activates MAPK.

Question 10

What did the data show?

Question 11

What could be interpreted from the data?

A) Inhibited MAPK

Explanation / Answer

10. PD had no effect on the percentage of neurons with BDNF-stimulated filopodia

11. MAPK inhibition is required for filopodia formation

A) Inhibited MAPK

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To test if MAPK was activated by BDNF, wild type neurons were treated with BDNF,

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