A and B On the graph, draw the best-fit line for these data points. You want to

Question

A and B On the graph, draw the best-fit line for these data points. You want to determine the protein concentration of some purified OFP protein using the Bradford assay. To do this, you measured the absorbance of the protein standards (shown on the graph) as well as the absorbance of your purified GFP protein sample. The absorbance of your GFP protein sample is 1.0 au. What is the approximate concentration of your purified GFP? show how you determined this on the graph, and write the correct units for the protein concentration.

Explanation / Answer

The best fit line can be drawn as a line connecting majority of the points in the graph.

The approximate concentration of GFP protein with absorbance 1.0 will have a concentration of 1 mg/ml. Since the absorbance corresponds to the concentration along the straight line at that particular concentration.

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