1) What is the complementary DNA sequence to 5 ATGCATCG 3? Enter only the nucleo

Question

1) What is the complementary DNA sequence to 5 ATGCATCG 3? Enter only the nucleotides, and put your answer in the 5’ to 3’ direction.

2) If 5 CGATGCAT 3 is the template DNA strand, what is the sequence of the non-coding strand? Enter only the nucleotides, and put your answer in the 5’ to 3’ direction.

3) If 5 CGATGCAT 3 is the template DNA strand, what is the sequence of the mRNA transcript? Enter only the nucleotides, and put your answer in the 5’ to 3’ direction.

4) The template strand of a molecule of DNA contains 22%C, 18%G, 32%A, 28%T.

a) What percentage of the non-template strand is C?

b) What percentage of the RNA transcript is G?

c) What percentage of the RNA transcript is T?

Explanation / Answer

1) The complementary sequence to the above said DNA sequence is 5'-CGATGCAT-3'.

2) Template strand - 5 CGATGCAT 3. It should be noted that both template strand and non-coding regions denotes the same strand in a DNA molecule. The template strand is the one that would actually be transcribed. The other sequence is called the coding strand. Hence, the sequence for the non-coding strand would be the same.

3) Template strand 5 CGATGCAT 3. It is the template strand that would be transcribed during transcription. The mRNA sequence to the template strand would be as follows.

Template strand - 5 CGATGCAT 3

mRNA strand - 5'-AUGCAUCG-3'

4) a) Since the template and non-coding strands denote the same the % of C remains the same (22%)

b) 22 % G that is complementary to the 22% C present in the template strand.

c) 0%. RNA are said to replace thymine with uracil in their configuration.

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