1. the procedure calls for vigorous grinding of the onion cells with sand. would

Question

1. the procedure calls for vigorous grinding of the onion cells with sand. would you be able to isolate the dna in a cells if this step were omitted? whats the purpose of the grinding process?

2. in the experiment, how did you minimize the interaction between the dna and histones(proteins)?

3. what coumpounds remain in the ethanol solution after the dna is removed by spooling onto a glass rod?

4. could you do without using the detergent in the procedure? explain your answer.

5. is th dephenylamine reagent able to distinguish between ribose and deoxyribose? could it also distinguish between DNA and RNA?

6. what is the structural difference between glucose and ribose? did the diphenylamine test work for the glucose solution?

Explanation / Answer

1.

No we cannot isolate the DNA in onion cells by omitting this step. The purpose of grinding these cells is to isolate the DNA. Since the onion cells have rigid cell wall, they need to be grind well so that the cell wall and plasma membrane rupture and release the cell components.            

5.

Yes diphenylamine reagent is able to distinguish between ribose and deoxyribose. The reaction between this reagent and deoxyribose produces blue color, while no color will be produced with ribose.

6.

Both ribose and glucose are monosaccharides, but they differ in number of carbons they have. Ribose has 5 carbon atoms, while glucose has 6 carbon atoms. Because of this difference, they also differ in melting point, molar mass, and density.

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