Suppose you are interested in discovering new human proteins that are involved i

Question

Suppose you are interested in discovering new human proteins that are involved in trafficking other proteins from the cytoplasm to the mitochondria (an essential process in cells remaining alive). However, your lab experience is in yeast. In precise detail, write an outline of an experimental design you would use for generating, verifying, and isolating a haploid yeast strain with a mutation in a key protein in this specific trafficking process, and then describe how you would use this yeast strain to identify, isolate, and ultimately sequence a human cDNA that encodes a protein essential in this trafficking mechanism. Please outline and include all important steps.

Explanation / Answer

Protein traffiking ; It is the process begins with the targeting of the proteins to their proper destinations ,These are carried out on the cis side of the memberane cytosolic and is co translational or shortly after synthesis of proteins on the ribosome . It is controlled by its signal sequence or lack of signal ; ER signal peptide ,mitochondria signal sequence ,choloroplast ER transit peptide .each signal sequence is different .

Yeast act as a best model for the studying the eukaryotic process as represents the most of the eukaryotic process .The secretory pathway is the best process involving first traffiking process .yeast cells are unicellular organisms It has shared complex intracellular organization with the higher eukaryotes .it has the short generation time of about 1.5hrs in the rich medium . Memberane and soluble organellar proteins are generally translocated ER during the symthesis.

Endocytosis is a process where we can see the traffiking of the proteins .They are mainly actin dependent based on the genetic screening ,allowing the isolation at the end .charecterization of these end mutants and other isolated in different genetic screening .Two different types of the yeast mutants were observed one include mutanant which can produce the actin protein and others which cannot produce the actin filaments ,actin related proteins ,actin polymerisation effectors WASP .these all proteins involve in the endocytic internalization step.

New traffiking process include vesicular transport between the organelles ,which plays an importanat process include cellular homeostatis . which include the contact sites between the mitochondria and ER .these exchange sites depend on the protein called ERMES .such interactions are found in the nuclus vacoule junction and allowing the pecimeal auto phagy of the nucleus and its degradation in the nucleus .Other protein include which act as a contact site include vacoule and mitochondria is V CLAMP . the deletion of the either ERMES or the V CLAMP causes the expasion of the system to complement loss of the function and deletion of the both is lethal .These shows that the highlet the role of the interorganellar contact sites in memberane traffiking

Related Questions

Navigate

• Browse (All)
• Browse S
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.