Actin-binding proteins bind to actin and can modify its properties. You purify a

Question

Actin-binding proteins bind to actin and can modify its properties. You purify a protein, Cap1, that seems to bind and cap one end of an actin filament, although you do not know whether it binds the plus end or the minus end. To determine which end of the actin filament your protein binds to, you decide to examine the effect of Cap1 on actin polymerization by measuring the kinetics of actin filament formation in the presence and the absence of Cap1 protein. You obtain the following results (see Figure Q5). Do you think Cap1 binds the plus end or the minus end of actin? Explain your reasoning.

Explanation / Answer

Do you think Cap1 binds the plus end or the minus end of actin? Explain your reasoning.

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