A plasmid lacking the ORI element would not be highly useful for gene cloning be

Question

A plasmid lacking the ORI element would not be highly useful for gene cloning because:

a. it would lack sufficient restruction sites for convenient insertion of the gene of interest

b.selection for cells containing this plasmid would not be possible.

c.transciption would fail to terminate properly leading to poor gene expression

d. the plamis would fail to replicate and remain stable in a proliferation cell population

explain your answer please

What amplification factor would result from a 35 cycle PCR reaction targeting a 1400 bp gene?

explain and show your work please

DNA ligase catalyzes hybridization for complementary sticky ends ( T or F)

cDNA synthesis often makes use of the polyA tail of euk mRNA for priming reverse transcriptase (T or F)

How do you determine whether or not an oligonucleotide is a good PCR primer?

Explanation / Answer

d. Plasmid would fail to replicate and remain stable in a proliferation cell population.

ORI or origin of replication functions to allow initiation of replication in a plasmid. If this ORI is absent then replication will not take place and stability would be affected.

False

DNA ligase does not catalyse the hybridization for complementary sticky ends but rather repairs the nicks caused due to hybridization by the formation of phosphodiester bonds.

True

For cDNA synthesis the transcript is primed with poly A tail of euk mRNA.

The determination that an oligonucleotide is a good PCR primer is based on factors like melting temperature Tm, absence of dimerization capacity and absence of hairpin formation. It also depends on lack of secondary primary sites in the template.

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