The growth of the HPV was monitored as follows: 10 9 cells and 10 6 viruses in o
ID: 64756 • Letter: T
Question
The growth of the HPV was monitored as follows: 109 cells and 106 viruses in one milliliter were incubated under appropriate conditions. Following a first infectious cycle, a direct count and a plaque assay were performed. In the case of the direct count, the mixture was diluted by a factor of 1 000X after which beads were added to obtain a final concentration of 1 X 104 beads/mL. A microscopic field of vision revealed 20 beads and 150 virions. In the case of the plaque assay, 67 plaques were observed on a plate on which 0.1 mL of a 10-5 dilution was assayed.
1. What was the initial multiplicity of infection?
2. According to the direct count, what was the volume observed in a field of vision?
3. According to the direct count, what was the number of virus/mL after the first infectious cycle?
4. According to the direct count, what was the burst size after one infectious cycle?
Explanation / Answer
Multiplicity of infection (MOI) is the number of virus particles added per cell. So , here 106 virus particles were added to 109 cells. So, MOI is equal to 106/109 ~ 1 (slightly less than 1)..
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