1. A single stranded DNA molecule (a template) is shown below: 5\'-AAAGGGTCAGTGA

Question

1. A single stranded DNA molecule (a template) is shown below:

5'-AAAGGGTCAGTGACCTAGGCGACCAACCTT-OH 3'

Which of the following short DNA sequences could act as a primer that would allow DNA polymerase to synthesize the complementary strand to the template? (Note: the answers are all written in the 5'--> 3' direction...you may need to flip them around to confirm whether they could act as primers for this tempalte.)

Select one:

a. 5'-AAGGTT-OH 3'

b. 5'-CCCTTT-OH 3'

c. 5'-AAAGGG-OH 3'

d. 5'-AACCTT-OH 3'

e. 5'-TTTCCC-OH 3'

2. A replication fork is shown below. The triangle with "H" represent helicase. The 5' and 3' ends of the two template strands are labeled. Do you have enough information to determine which strand (A or B) will be the template for leading strand synthesis?]

Select one:

a. Yes...strand A is the template for the leading strand.

b. Yes...strand B is the template for the leading strand.

c. No...more information is needed to determine which is the template for the leading strand.

Explanation / Answer

1. The DNA polymerase extends the DNA template in the 5'------> 3'. So the binding of the primer to the template DNA should be in the direction of 3'-----> 5' direction.

5'-AAAGGGTCAGTGACCTAGGCGACCAACCTT-OH 3'

3' TTGGAA 5'

The answer is option a

2. More information is needed as I cant see the figure you have described about.

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