An in vitro system was developed to study actin assembly and disassembly in nonm
ID: 64142 • Letter: A
Question
An in vitro system was developed to study actin assembly and disassembly in nonmuscle cells. In this study, tissue culture cells were incubated for several hours with [35S]methionine so that all the actin monomers in each filament were labeled. Actin filaments were then collected by differential centrifugation and placed in a buffer containing one of three cytosolic extracts (A, B, or C). The amounts of soluble actin in each sample were monitored over time as shown in the following graph. What do these results tell you about the effects of A, B, and C on the assembly and disassembly of actin filaments?
If someone could please save my life and explain this I would be eternally grateful!
An in vitro system was developed to study actin assembly and disassembly in nonmuscle cells. In this study, tissue culture cells were incubated for several hours with [35S] methionine so that all the actin monomers in each filament were labeled. Actin filaments were then collected by differential centrifugation and placed in a buffer containing one of three cytosolic extracts (A, B, or C). The amounts of soluble actin in each sample were monitored over time as shown in the following graph. What do these results tell you about the effects of A, B, and C on the assembly and disassembly of actin filaments?Explanation / Answer
The effect of B on actin can concluded that it is showing no effect which can de deduced by equilibrium between polymerisation and depolymerisation of actin monomers. From the graph you can see that there is constant radioactivity with increase in time.
The effect of C on actin can concluded that it is inhibiting the polymerisation of actin monomers into a filament. This is evident by constant basal levels of radioactivity with increase in time
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