1. Use the Diagram below to answer part a to c: A 15-nt oligonucleotide was used
ID: 63618 • Letter: 1
Question
1. Use the Diagram below to answer part a to c:
A 15-nt oligonucleotide was used as the sequencing primer. The bottom of the gel represents the positive-pole during the electrophoresis of the samples.
a) What is the DNA sequence of this gene starting from the 3'-end of the primer (1st 10-nts only)?
options:
a) 5'-ATCATCAGCA
b) 5'-GACGACGACG
b) For the nucleotide marked with the asterisk, what is the length of this nucleotide?
options:
a) 0
b) 1
c) 2
d) 13
e) 14
f) 28
g) 29
c) For the nucleotide marked with the asterisk, how many ddNTPs are present in the DNA fragments found in this band?
options:
a)
0
b)
1
c)
2
d)
13
e)
14
f)
28
g)
29
Use the Diagram below to answer questions 2 part a to d:
2.
a) If there are 5 different VNTR alleles at the VNTR63 locus, then how many genotypes would be possible in the population (ie V63-1/V63-2 would be one possible genotype)?
options:
a
5
b
10
c
15
d
20
e
25
f
70
b) If you perform PCR analysis of this region using the PL and PR primers, how many bands will you see on a gel from an individual with the following genotype:
V63-2/V63-2
options:
a
0
b
1
c
2
d
3
e
4
f
70
c) If you perform PCR analysis of this region using the PL and PR primers, how many bands will you see on a gel from an individual with the following genotype:
V63-4/V63-5
options:
a
0
b
1
c
2
d
3
e
4
f
70
d) If you perform PCR analysis of this region using the PL and PR primers, how many bands will you see on a gel from an individual with the following genotype:
V63-1/V63-2
options:
a
0
b
1
c
2
d
3
e
4
f
70
d) What will be the size of the DNA fragment in base pairs (bp) following amplification of V63-4 with the PL and PR primers as shown in the diagram on your worksheet (Q6)?
options:
a
10
b
30
c
40
d
50
e
70
f
80
e) The line below represents a mature eukaryotic mRNA. Place the following list of items in the proper order that you would find them on the mature mRNA. Note: some of the items may NOT be found on this mRNA.
5’--------------------------------------------------------------------------------------------3’
List of items:
Items to be ordered on the mRNA:
stop codon
poly-A tail
intron
3'-UTR
promoter
start codon
Kozak sequence
5'-cap
Shine-Delgarno Sequence
5'-UTR
Note: not all of the items may be on this particular mRNA
a) 5'-ATCATCAGCA
b) 5'-GACGACGACG
Explanation / Answer
a). a) 5'-ATCATCAGCA
In a gel we read the sequenc efrom bottom to top, which gives 5' to 3' direction.
b). e. 14
The nucleotide marked with the asterisk, is at 14th position (count from the bottom).
c). e. 14
Each ddNTP (dideoxynycleotide phosphate) contains one nucleotide, so for the nucleotide marked with the asterisk, 14 ddNTPs are present in the DNA fragments found in this band.
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