1. A viral genome was sequenced and the following percentages of nucleotides wer
ID: 63611 • Letter: 1
Question
1. A viral genome was sequenced and the following percentages of nucleotides were observed:
Virus 2: A=20%, C=30%,G=30%,U=20%
On what type of molecule is the genetic information contained for Virus 2?
options:
a
dsDNA
b
ssDNA
c
dsRNA
d
ssRNA
A 15-nt oligonucleotide was used as the sequencing primer. The bottom of the gel represents the positive-pole during the electrophoresis of the samples.
2.What is the DNA sequence of this gene starting from the 3'-end of the primer (1st 10-nts only)?
options:
a) 5'-ATCATCAGCA
b) 5'-GACGACGACG
3. For the nucleotide marked with the asterisk, what is the length of this nucleotide?
options:
a) 0
b) 1
c) 2
d) 13
e) 14
f) 28
g) 29
4. For the nucleotide marked with the asterisk, how many ddNTPs are present in the DNA fragments found in this band?
options:
a)
0
b)
1
c)
2
d)
13
e)
14
f)
28
g)
29
5. If there are 5 different VNTR alleles at the VNTR63 locus, then how many genotypes would be possible in the population (ie V63-1/V63-2 would be one possible genotype)?
Question 5 options:
a
5
b
10
c
15
d
20
e
25
f
70
6. If you perform PCR analysis of this region using the PL and PR primers, how many bands will you see on a gel from an individual with the following genotype:
V63-2/V63-2
Question 6 options:
a
0
b
1
c
2
d
3
e
4
f
70
7. If you perform PCR analysis of this region using the PL and PR primers, how many bands will you see on a gel from an individual with the following genotype:
V63-4/V63-5
Question 7 options:
a
0
b
1
c
2
d
3
e
4
f
70
8. If you perform PCR analysis of this region using the PL and PR primers, how many bands will you see on a gel from an individual with the following genotype:
V63-1/V63-2
Question 8 options:
a
0
b
1
c
2
d
3
e
4
f
70
9. What will be the size of the DNA fragment in base pairs (bp) following amplification of V63-4 with the PL and PR primers as shown in the diagram on your worksheet (Q6)?
Question 9 options:
a
10
b
30
c
40
d
50
e
70
f
80
10. The line below represents a mature eukaryotic mRNA. Place the following list of items in the proper order that you would find them on the mature mRNA. Note: some of the items may NOT be found on this mRNA.
5’--------------------------------------------------------------------------------------------3’
List of items:
Items to be ordered on the mRNA:
stop codon
poly-A tail
intron
3'-UTR
promoter
start codon
Kozak sequence
5'-cap
Shine-Delgarno Sequence
5'-UTR
Note: not all of the items may be on this particular mRNA
a
dsDNA
b
ssDNA
c
dsRNA
d
ssRNA
Explanation / Answer
1). C. dsRNA
The DNA (deoxyribonucleic acid) nucleotides otherwise called “bases” carry the message encoding for the synthesis of a protein. There are four such bases, namely, adenine (A), thymine (T), guanine (G), and cytosine (C), which are the building blocks of DNA’s double helical structure. DNA do not contain uracil (U), it is the component of RNA (ribonucleic acid). In RNA, uracil is present in place of thymine.
Among these base pairs, adenine binds with thymine and guanine binds with cytosine, this is called as complementary pairing.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.