The effects of a poison on actively respiring mitochondria are being studied to

Question

The effects of a poison on actively respiring mitochondria are being studied to determine its mode of action. When the poison is added, every component of the electron transport chain is found to be in its reduced form based on spectroscopic analysis, and ATP production ceases entirely. It is reasoned that the poison is either blocking the final transfer of electrons to oxygen or is blocking the ATP synthase. Which of the following experiments will help unravel this dilemma?

Add a second blocking agent to interfere with either Complex I or Complex II along with the poison.

Add an artificial electron donor along with the poison.

Add an uncoupling agent along with the poison.

There is no justification to come to that conclusion in the first place.

None of the above will help distinguish between these two possibilities.

Add a second blocking agent to interfere with either Complex I or Complex II along with the poison.

Explanation / Answer

the second option

Add an artificial electron donor along with the poison. will be the best experiment beacuse if its blocking the final transfer of electron then adding poison withelectron doner will have no effect on the mitocondria (there is a artificial electron doner ) if the poison actually blocking the ATP synthesis then miticondria will be effected

Add an artificial electron donor along with the poison. will be the best experiment beacuse if its blocking the final transfer of electron then adding poison withelectron doner will have no effect on the mitocondria (there is a artificial electron doner ) if the poison actually blocking the ATP synthesis then miticondria will be effected

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