3-HBA exists as two different stereoisomers, D-3-HBA and L-3-HBA. The kinetics o
ID: 63128 • Letter: 3
Question
3-HBA exists as two different stereoisomers, D-3-HBA and L-3-HBA. The kinetics of transport of these stereoisomers through the blood brain barrier can be determined by adding radiolabeled (C14) L-and D- 3-HBA into the blood and measuring the velocity of uptake of radiolabeled C14 3-HBA into the brain (see the figure on the left). What conclusions about 3-HBA transport through the blood brain barrier can be made from examining these kinetic data? Be specific in regard to the type of transporter (channel or carrier), the specificity of transporter (affinity for D-3-HBA and L-3-HBA), and the shape of the curves? Explain your answers.
E E 0.8 D737HBA' L737-BA" 0.2 0.0" o"2o'"""40""""60'"80"100" Blood'levels'of'3BA"Explanation / Answer
1. It is a carrier (Facilitated transport) as the graph is hyperbolic and gets saturated.
2. From the curves, the higher velocity at same substrate concentration at steady state (plateau) region shows that the carrier has higher affinity for D-3-HBA.
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