Aminotransferases are enzymes that catalyze the interconversion of amino acids a

Question

Aminotransferases are enzymes that catalyze the interconversion of amino acids and ?-keto acids. In this way, they can provide the carbon needed for gluconeogenesis from amino acids supplied by dietary protein. For example, aspartate aminotransferase and alanine aminotransferase catalyze the reactions shown below. The equilibrium constant for the aspartate aminotransferase reaction is 0.147 If a cell contains 30 mM aspartate, 0.44 mM ?-ketoglutarate, 0.1 mM OAA and 9.6 mM glutamate, how much OAA will be formed when the aspartate aminotransferase reaction comes to equilibrium? Asp AT Asp + a-ketoglutarate --> OAA + Glu Ala AT Ala + a-ketoglutarate --> pyruvate + Glu

Explanation / Answer

Asp AT Asp + a-ketoglutarate --> OAA + Glu Ala AT Ala + a-ketoglutarate --> pyruvate + Glu

30 mM 0.44 mM 0.1 mM 9.6 mM

let x mole reacted so xmole will be formed

30 mM-x 0.44 mM-x 0.1 mM+x 9.6 mM +x

now Ka=0.147

thus (0.1+x)(9.6+x)/(30-x)(0.44+x)=0.147

thus on solving for x we get

0.1+x=0.46)(0.44+x)

thus x=0.07

thus 0.07 milli mole will be formed

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