Based on these results: 1. Give a short description of what you would see for gr

Question

Based on these results: 1. Give a short description of what you would see for growth on the plates if both antibiotic resistances (amp and strep) resided vi the genomic DNA instead of the plasmid? 2. What is the drug resistance of strain I? Strain II? 3. Which plate shows growth in the mating sector that cannot be explained by either strain alone? Assume that one antibiotic resistance gene has been transferred. What are the possible transfers? 4. On which plates do you expect your test colonies to grow? On which plates are you unsure? Are there plates on which you don?t expect growth? Why? 5. Describe the donor and recipient strains as well as any gene transfer that has occurred and indicate which observations led you to this conclusion.

Explanation / Answer

1. If amp and strep antibiotic resistance resides in the genomic DNA instead of plasmid, then bacteria would grow on both amp and strep added plates. But bacteria containing amp resistance plasmid would grow on amp added plate only but not on strep added plate. Likewise, bacteria containing strep resistance plasmid would grow on strep added plates only.

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