Having some issues comprehending what the assignment is talking about. We figure
ID: 62764 • Letter: H
Question
Having some issues comprehending what the assignment is talking about. We figured that growth factor depletion would result in the number of cells increasing in the first portion of part B, but we are having issues with answering why growth factor depletion synchronizes cells. In addition, we are also confused on how we could synchronize cells in prometaphase with a method and what the subsequent histogram would look like.
1) A histogram of a typical, freely dividing cell culture is shown in (A) (B) Growth factor depletion can be used to "synchroniz of the cell cycle. Show what the resulting histogram would look like following growth factor depletion. Why would growth factor depletion synchron factor depletion can be used to "synchronize" cells such that they are all in the same stage metaphase and a method or manipulation that could be used to synchronize cells in pro (C) Propose draw the resulting histogram for these cells (A) 200 160 120 80 S 40 200 400 600 Fluorescence per cell (a.u.) 200 160 120 200 160 120 80 S 40 S 40 0 0 200 400 600 200 400 600 Fluorescence per cell (a.u.) Fluorescence per cell (a.u.)Explanation / Answer
(B)
* When an essential nutrient concentration is limited, growth of all the cells is arrested at a particular stage. The cells which are now in synchrony can then be induced to carry on the cell cycle. All the cells will be at same stage of growth and grow synchronously then.
* Cells can be synchronized by arresting cell cycle at prometaphase by using a number of chemicals. One such chemical is nocodazole. Nocodazole treated cells enter mitossi but cannot form metaphase spindles. Nocodazole blocks the polymerization of microtubules, and thus inhibit spindle assembly.
Other drugs are colcemid, vincristine, with similar effects.
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