Background for questions 15-17. From the bacterium Staphylococcus aureus you hav
ID: 62363 • Letter: B
Question
Background for questions 15-17. From the bacterium Staphylococcus aureus you have isolated a gene encoding an antibiotic-degrading protein along with its promoter. You plan to isolate mutants with alterations in the promoter to explore its regulation and interaction with RNA polymerase.
15. What would be the most likely consequence on transcription of the antibiotic-degrading gene of changing all the A-T pairs in the sequence 10-bases upstream from the mRNA transcription start site to G-C pairs?
A. Increase transcription
B. Decrease transcription
C. Would not influence transcription
16. What would be the most likely consequence on transcription of the antibiotic-degrading gene of substituting all the A-T base pairs with the same number of G-C base pairs in the sequence between the -10 and -35 conserved sequences?
A. Increase transcription
B. Decrease transcription
C. Would not influence transcription
17. What would be the most likely consequence on transcription of the antibiotic-degrading gene of deleting five bases pairs in the sequence between the -10 and -35 conserved sequences?
A. Increase transcription
B. Decrease transcription
C. Would not influence transcription
*EXPLANATIONS WOULD BE AWESOME*
Explanation / Answer
15)B
-10 sequence has conserved sequence of TATAAT..its important for interactiin with sigma factor. RNA polymerase
16) c
Sequence between _10 and -35 is not conserved varies from promoter to promoter
17)c
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