41. Integrase A. inserts the double-stranded retrovirus RNA into the host genome

Question

41. Integrase A. inserts the double-stranded retrovirus RNA into the host genome. B. inserts the double-stranded retrovirus DNA into the host genome. C. removes the retrovirus RNA from the host genome D. removes the double-stranded retrovirus DNA from the host genome. E. B and D For questions 42-44 all plates have glycerol as an energy and carbon source that does not interfere with functional -galactosidase expression of the lac operon. All plates also have X-gal. All bacteria have a gene (Z*) in the lac operon. The rest of the genoty pe of the ba of the bacteria, and whether the plates have inducer (IPTG) and/or glucose, are indicated in each question. All genes are wild type unless noted otherwise. 42. When the plates have inducer (IPTG) and glucose, blue colonies will be produced by A. Wild type bacteria. B. bacteria C. O bacteria. D. CAP bacteria E. None of the above 43. When the plates have inducer (IPTG) and NO glucose, white colonies will be produced by A. Wild type bacteria B. I bacteria C. O bacteria. D. CAP bacteria E. A, B and C 44. When the plates have NO inducer (IPTG) and NO glucose, blue colonies will be produced by A. Wild type bacteria. B. I bacteria. C. Or bacteria. D. CAP- bacteria E. Both B and C 45. Imagine you grew a liquid culture of E coli cells, which are wild type for ALL loci, and tested for constitutive expression of particular genes. Which of the following genes are expressed constitutively when the E coli cells are grown in a solution with glucose and inducer (glucose levels remain high during the study). A. Lac I B. Lac Z C. Lac Y D. All of the above E. Only B and C 46. The most common way a cell prevents the movement of autonomous retroviruses and DNA transposons is by A. preventing their transcription. B. preventing translation of their RNAs C. inhibiting the activity of their enzymes. D. cutting them out of the genome with restriction endonucleases E. all of the above.

Explanation / Answer

41. Option -B. Integrates double stranded retrovirus DNA into host genome.

42. Wild type bacteria - It will have production of beta galactosidase upon induction by inducer IPTG and converts the plate into blue.

43 O- bacteria, because when there is no glucose and inducer it should take up x-gal and produce beta galactosidase. Since the operon is not available it is not able to produce the enzyme.

44. Both b and c. Since there is no operator and repressor it produces blue colonies even in the absence of glucose and inducer.

45. Only B and C. It will help in lactose intake and cleavage.

46. All the above. - prevention is mediated by avoiding its insertion, transcription and translation

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