17. You are working with a genomic DNA library. This library contains human geno

Question

17. You are working with a genomic DNA library. This library contains human genomic DNA cloned into the pc DNA plasmid. You want to insert your genomic DNA containing pc DNA recombinant plasmids into bacteria, then purify the plamid and determine the DNA sequence. But you don ?t make sure that each plamid enters a single bacterium. (You use too many plasmids per bacteria). You then clone a single bacterium with recombinant DNA. purify plasmid DNA and perform chain - termination sequencing reaction. The result is shown below. When you look at this sequencing result you conclude that you have two different plasmids in this bacterial clone but not 3 or 4. How do your reach this conclusion? If your bacteria contained 3 different plasmids. how would your results be? Please explain your logic briefly.

Explanation / Answer

If the bacteria contained single plasmid then you would find only one base at a particular position, as the addition of the dideoxy base would stall the reaction. In the reaction above the bacteria is having two plasmids which can be proved by the presence of two bases at a single position. For example at position 9, you can find both A and T and in some positions you find only one base for example at position 10, there is only presence of A.

If the bacteria are having more than 2 plasmids, then in the reaction above you might some positions where there would more 2 bases in a particular position. This would prove that bacteria is having more than 2 plasmids.

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.